Part:BBa_K187001:Experience

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Please enter how you used this part and how it worked out.

Applications of BBa_K187001

This part has been tested extensively by the BioBytes team, and has worked as expected in all assays. See the Alberta wiki for more information: http://2009.igem.org/Team:Alberta. The plasmids pBA and pAB have been used in all assays for the bead assembly method, as described in the BioBytes BBF RFC.

Briefly, Biobytes is a new standardized in vitro assembly method. The system is based on a bead platform where constructs with long sticky ends are annealed together sequentially. This system is designed to enable the single day assembly of plasmids composed of up to about half a dozen premade parts. Complementary to existing BioBrick standards for DNA assembly, we expect this system to be useful for rapid generation of large constructs wherever an interpart spacing of 37 bp can be tolerated.

User Reviews

UNIQ43d2cc847161770c-partinfo-00000000-QINU UNIQ43d2cc847161770c-partinfo-00000001-QINU